Canola Data

Our population is derived from the doubled haploid line DH12075.

We usually screen amplicons of 1500 - 1800 base pairs, but this depends on the length of genomic sequence avaialble to design primers

We obtain, on average, more than 20 mutations per gene (~42% missense, 4% nonsense, and 54% silent)

Data from our project are below (please click on the amplicon name to download the data file for that locus):

Amplicon	Gene	Number of Mutations	Lines Screened	Amplicon Size (bp)	Mutation Density*
bn1	Stearoyl-acyl carrier protein desaturase	21	1152	1505	1/72
bn2	Acyl-ACP thioesterase A	8	1152	1534	1/192
bn3	β-ketoacyl-ACP synthetase II	28	1920	1579	1/95
bn4	Lycopene epsilon cyclase	22	1536	1813	1/112
bn5	Acyl-ACP thioesterase B	20	1152	1655	1/84
bn6	Dihydroflavonol reductase	13	1536	1596	1/165
bn7	GLABRA 2 homologue	21	1152	1748	1/85
bn9	Phosphatidylinositol-dependent phospholipase 2	26	1536	1377	1/70
bn13	Lysophosphatidylcholine acyltransferase	11	1152	1689	1/156
bn14	Lysophosphatidylcholine acyltransferase	21	1536	2100	1/139
bn15	TRANSPARENT TESTA 16 homologue	17	1920	1605	1/159
bn17	Myo-inositol phosphate synthase	38	1536	1583	1/56
bn19	TRANSPARENT TESTA 16 homologue	12	1152	1695	1/143
bn20	DE-ETIOLATED homologue	9	1152	1702	1/192
bn21	Diglyceride acyltransferase 1	10	1536	1181	1/150
bn22	Diglyceride acyltransferase 1	2	384	1805	1/308
bn23	Diglyceride acyltransferase 1	29	1536	1798	1/85
bn25	BREVIPEDICELLUS homologue	10	1152	1306	1/127
bn26	BREVIPEDICELLUS homologue	12	1152	1739	1/148
bn27	MUCILAGE MODIFIED 4 homologue	7	1152	1761	1/257
bn29	Acyl-ACP thioesterase A	17	1152	1487	1/87
bn30	Acyl-ACP thioesterase B	14	1152	1392	1/98
bn31	β-ketoacyl-ACP synthetase II	17	768	1457	1/57
bn36	FUSCA 3 homologue	15	1536	1781	1/162
bn37	WRINKLED 1 homologue	12	768	1782	1/101
bn39	HIGH-LEVEL EXPRESSION OF SUGAR-INDUCIBLE GENE 2 homologue	20	1536	1391	1/91
Average:		17	1285	1618	1/109

*Total number of mutations divided by the total number of kb of DNA screened. The amplicon size used for this calculation was reduced by 200bp as the ends of the amplicons cannot be screened using the LI-COR detection system

For more information please contact us by email

Last updated 13-09-11 by Erin Gilchrist.